a5040 biotin peg4 alkyne click chemistry tools (Click Chemistry Tools)
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Click Chemistry Tools
a5040 biotin peg4 alkyne click chemistry tools
A5040 Biotin Peg4 Alkyne Click Chemistry Tools, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin-peg4-alkyne/nhs+peg4+reagent+tco/pm40956668-135-79-81
Average 86 stars, based on 1 article reviews
A5040 Biotin Peg4 Alkyne Click Chemistry Tools, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin-peg4-alkyne/nhs+peg4+reagent+tco/pm40956668-135-79-81
Average 86 stars, based on 1 article reviews
a5040 biotin peg4 alkyne click chemistry tools - by Bioz Stars,
2026-09
86/100 stars
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Clinical Proteomics:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Recombinant:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Whole Genome Amplification:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Protease Inhibitor:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Membrane:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Activity Assay:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Luciferase:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Plasmid Preparation:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Polymerase Chain Reaction:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Purification:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Bicinchoninic Acid Protein Assay:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 Western Blot:Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: Article Title: Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling. Article Snippet: Clickable unnatural sugars have been widely used in studying glycosylation in living systems via the metabolic glycan labelling (MGL) strategy.. Partial protection of unnatural sugars by 1,6-di-O-acylation increases the labelling efficiency while avoiding the non-specific S-glyco-modification.. Herein, we report the facile synthesis of a series of clickable unnatural sugars in both the unprotected and 1,6-di-O-acylated forms at the ten-gram scale. Article Title: N 6 -adenomethylation of GsdmC is essential for Lgr5 + stem cell survival to maintain normal colonic epithelial morphogenesis. Article Snippet: Article N6-adenomethylation ofGsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis Article Title: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis. Article Snippet: RNA molecules with the expanded CAG repeat (eCAGr) may undergo sol–gel phase transitions, but the functional impact of RNA gelation is completely unknown.. Here, we demonstrate that the eCAGr RNA may form cytoplasmic gel-like foci that are rapidly degraded by lysosomes.. These RNA foci may significantly reduce the global protein synthesis rate, possibly by sequestering the translation elongation factor eEF2. Article Title: FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. Article Snippet: Short article Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis Article Snippet: 5mM Article Title: Antibody-immunotoxin Conjugate Using FcBP-mediated Photoconjugation to Treat Cancer. Article Snippet: Background/Aim: Cytotoxic payload conjugation to antibodies efficiently suppresses tumors and contributes to the improvement of cancer survival.. In our previous study, c-Kit targeting antibody–drug conjugate (2G4-DM1) with DM1, a microtubule inhibitor, efficiently suppressed tumor growth.. However, slow-growing c-Kit-positive tumors, such as GIST-48, did not efficiently respond to DM1. Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT . Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9102S; RRID: AB_330744 Anti-P-p44/42 MAPK (Erk 1/2) Cell Signaling Technology Cat# 9101S; RRID: AB_331646 Anti-alpha/beta-Tubulin Cell Signaling Technology Cat# 2148S; RRID: AB_2288042 IRDye 800 CW Streptavidin LI-COR Cat# 926-32230 IRDye 800 CW Goat anti-Rabbit LI-COR Cat# 926-32211; RRID: AB_621843 Anti-Pax7 Developmental Studies Hybridoma Bank, U of Iowa PAX7; RRID: AB_2299243 Anti-Plg-RKT ThermoFisher Cat#PA5-98932; RRID: AB_2813545 Alexa Fluor 647 Invitrogen Cat#A32733; RRID: AB_2633282 Anti-eMHC_BF-G6 Developmental Studies Hybridoma Bank, U of Iowa Cat#BF-G6; RRID: AB_10571455 Anti-GFP Cell Signaling Technology Cat#2555; RRID: AB_10692764 APC-CD45 Biolegend Cat#157605: RRID: AB_2876537 APC-CD11b Biolegend Cat#101211; RRID: AB_312794 APC-CD31 Biolegend Cat#102410; RRID: AB_312904 APC-Ter119 Biolegend Cat#116212; RRID: AB_313713 FITC-Sca1/Ly6a Biolegend Cat#108105; RRID: AB_313342 PE-VCAM1/CD106 Biolegend Cat#105713; RRID: AB_1134164 Anti-rabbit PE secondary ThermoFisher Cat#P-2771MP; RRID: AB_2539845 Anti-rabbit Alexa FlourTM 647 Plus secondary ThermoFisher Cat#A32733; RRID: AB_2633282 Anti-mouse FITC secondary ThermoFisher Cat#F-2765; RRID: AB_2536525 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 HyperfilmTM ECLTM Sigma-Aldrich Cat#GE28-9068-36 Biological samples CALERIETM plasma CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM muscle biopsies CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf CALERIETM adipose tissue CALERIETM Bio repository https://calerie.duke.edu/sites/default/ files/2022-05/phase2_protocol.pdf Chemicals, peptides, and recombinant proteins DAPI ThermoFisher Cat#62248 EdU Sigma-Aldrich Cat# 900584 WGA 555 ThermoFisher Cat#W32464 |